Recombinant TNF alpha (Tumor necrosis factor alpha), Mouse, AF

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Recombinant TNF alpha (Tumor necrosis factor alpha), Mouse, AF
SDS- PAGE analysis of recombinant mouse TNF alpha
Recombinant TNF alpha (Tumor necrosis factor alpha), Mouse, AF
Recombinant TNF alpha (Tumor necrosis factor alpha), Mouse, AF
Recombinant TNF alpha (Tumor necrosis factor alpha), Mouse, AF
Recombinant TNF alpha (Tumor necrosis factor alpha), Mouse, AF
Recombinant TNF alpha (Tumor necrosis factor alpha), Mouse, AF
Recombinant TNF alpha (Tumor necrosis factor alpha), Mouse, AF
Recombinant TNF alpha (Tumor necrosis factor alpha), Mouse, AF
Recombinant TNF alpha (Tumor necrosis factor alpha), Mouse, AF
Recombinant TNF alpha (Tumor necrosis factor alpha), Mouse, AF
Recombinant TNF alpha (Tumor necrosis factor alpha), Mouse, AF
Recombinant TNF alpha (Tumor necrosis factor alpha), Mouse, AF

产品简介

产品简介

Catalog No.

PY2589

Source

Mouse Cytokines

Reactivity

Measure by its ability to induce cytotoxicity in L929 cells in the presence of actinomycin D. The ED50 for this effect is <40 pg/mL. The specific activity of recombinant mouse TNF alpha is approximately >2.5x 107 IU/mg.

Application

Cell culture, Elisa

Calculated Mol Weight

18 kDa

Observed Mol Weight

16 kDa

Recommended Dilution

It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

Alternative Names

TNFSF2, Cachectin, Differentiation-inducing factor (DIF), Necrosin, Cytotoxin, TNS_x0002_F1A, TNF-a

Host

Escherichia coli

Isotype

Clonality

Modification

His-tag at the C-terminus

Immunogen

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Purification

>98% as determined by SDS-PAGE. Ni-NTA chromatography

Concentration

Buffer System

<0.1 EU per 1 μg of the protein by the LAL method. The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 8.0.

Storage Condition

Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months.

Description

TNF-α is a kind of pleiotropic pro-inflammatory cytokine. It is secreted by various cells, such as adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. Proteolysis of the integral membrane precursor form of TNF-α from cells soluble can release homotrimeric TNF-α. TNF-α can bind with some TNF-α receptors induces apoptosis, besides, also trigger other responses depending on cell type, receptor expression, and signal transduction status. TNF-α participate in the inflammatory response.

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PY2589

规格:

5μg, 20μg, 100μg, 500μg, 1mg

价格:

¥800/5μg, ¥2000/20μg, ¥6000/100μg, ¥18000/500μg, ¥26000/1mg

货期:

3-4天

订购信息:

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